Egg albumin and milk mixture as a new storage medium to preserve vitality of periodontal ligaments of intentionally extracted animal teeth

Aim: This study aimed to evaluate egg albumin and milk mixture as a new storage medium to preserve vitality of PDL of avulsed teeth. Materials and Media: 180 incisors of 45 New Zealand rabbits were atraumatically extracted and divided into 4 equal groups (45 teeth). Gr.1 and 3 teeth were equally divided into 3 subgroups (15 teeth for each) and stored immediately post extraction in HBSS & EAMM for 1, 4 & 6h respectively. By the same manner Gr.2 and Gr. 4 were equally divided and stored after 1h dry time in HBSS & EAMM for 1, 4 & 6h respectively. Then the teeth were removed and prepared for histological investigation & digital counting of PDL cells using trypan blue exclusion technique. Results: Teeth stored in HBSS immediately showed that 65.75%, 48.25% & 38.08% of PDL cells were vital after 1, 4 & 6h storage time respectively, while EAMM reported 49%, 50.06 & 47.67% for the same storage times. On the other hand teeth stored in EAMM after 1h dry time showed that 30.672% , 34.665% And 36.011% of PDL cells were vital after 1, 4 & 6h storage time respectively, while EAMM reported 45.582%, 50.79% and 45.78% for the same storage times Conclusion: EAMM has proved powerful higher results than HBSS in all storage times (1, 4 and 6 h) in 1 h dry time which is an evidence of its ability of replenishment of metabolites in depleted PDL cells and it can be used at trauma sites specially when immediate replantation is not available.