Bone Marrow Mesenchymal Stem Cells- Loaded Chitosan/Bioglass Scaffold For Reconstruction Of Segmental Bone Defect

Document Type : Original Article

Authors

1 Oral and Maxillofacial Surgery

2 Oral and maxillofacial surgery, Faculty of oral and dental medicine, Azhar University

3 Veterinary, Veterinary medicine, Cairo University

4 Tissue Culture and Cytology, National cancer institute

Abstract

ABSTRACT
Objectives: To reconstruct a critically sized segmental bony defect using chitosan/ bioactive glass scaffold seeded with osteogenically differentiated stem cells with the evaluation of the engineered bone tissue. Materials and methods: 18 adult male mongrel dogs were selected to be used in the present study. The experimental protocol was divided in to five stages; A. Teeth extraction. B. Bone marrow aspiration. C. Stem cells isolation and culturing. D. Scaffold preparation and seeding of BMSCs. E. Implantation of the engineered construct in the defect site. F. Evaluation of the newly formed osseous tissue. Results: Radiographic evaluation of the constructed tissue revealed the highest bone density (259.23 +/- 52.42 %) at week 16 by group I2. H& E stained engineered bone showed various stages of bone development where the newly formed bone was integrated with the host bone. Histomorphometric analysis revealed the increase in areas of mature newly formed bone by increasing time intervals. The highest mean bone area fraction value (47.72+/-1.94%) was recorded by group I2 at 16week time interval. Conclusion: This study suggests that chitosan/ bioglass tissue engineering scaffolds seeded with BMSCs could be a reliable treatment modality for reconstruction of critically-sized bone defects.
Keywords: Chitosan; Bioglass; Bone marrow stem cells; segmental defect

Keywords

Al-Azhar Journal of Dental Science
Volume 26, Issue 4 - Serial Number 4
October - Oral Medicine & Surgical Sciences Issue (Oral Medicine, Oral & Maxillofacial Surgery, Oral Pathology, Oral Biology)
October 2023
Pages 493-501

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