Evaluation of Antibacterial Effect of Silver Diamine Fluoride and Sodium Fluoride Varnish on Bacterial Biofilm (An In Vitro Study)

Document Type : Original Article

Authors

1 Pedodontics, dental medicine, Al-azher university, Cairo, Egypt

2 Department of Pedodontics and Oral Health Faculty of Dental Medicine, Boys, Cairo Al-Azhar University

3 Oral health and pedodontics department, faculty of dental medicine, Al Azhar University, Cairo

4 Microbiology department ,Faculty of Medicine, Girls, Cairo ,Al-Azhar University.

Abstract

Objectives: The present invitro study was carried to evaluate the antibacterial effect of silver diamine fluoride 38% (SDF) and sodium fluoride varnish 5% (NaF) on inhibition of bacterial biofilm formation subjects and methods: a total number of 48 biofilms were classified into three main groups (16 for each) and treated with. Group A: SDF 38% for 1 minute only per day for three consecutive days and biofilms were washed 3 times with fresh media. Group B: NaF Varnish 5% for 1 minute only per day for three consecutive days and biofilms were washed 3 times with fresh media. Group C: left untreated (control group). After treatment, fresh media was added to biofilms that were grown at 1st day and at 3rd day . Streptococcus mutans (S. mutans ) and Lactobacillus acidophilus (L. acidophilus) were grown anaerobically. Colonies were counted and colony forming unit (CFU) per mL of was calculated. Results: There was a statistically significant difference in the count of S. mutans and L. acidophilus between all groups in which the highest bacterial count was found in Group C group followed by Group B while the least bacterial count was found in Group A. Thus results recorded SDF (82%,83%) higher reduction than NaF (70%,74%) according to S.mutans at 1st and 3rd day respectively and SDF (89%,88%) higher reduction than NaF (74%,81%) according to L acidophilus at 1st and 3rd day respectively. Conclusion: SDF is a non-invasive, simple and low-cost approach to arresting dental caries.

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Al-Azhar Journal of Dental Science
Volume 27, Issue 2 - Serial Number 2
April - Pediatric Dentistry & Orthodontics Issue (Pediatric Dentistry, Orthodontics)
April 2024
Pages 261-267

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