Tumour volume and s-phase fraction analysis in induced hamster buccal pouch carcinoma utilizing cetuximab immunotherapy.

Document Type : Original Article

Authors

1 Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Palestine

2 Oral and Dental Pathology Department, Faculty of Dental Medicine (Boys- Cairo), Al-Azhar University, Egypt.

3 Oral pathology dept.Faculty of dental medicine, AlAzhar University, Cairo, Egypt

4 professor of Oral and Dental Pathology Department, Al-Azhar University (Boys), Cairo,Egypt

Abstract

Objective: This study aimed to investigate tumor volume and synthesis phase fraction (SPF) analysis after cetuximab treatment on induced hamster buccal pouch (HBP) carcinoma.
Material and Methods: 40 male Syrian hamsters were divided into 4 groups (G(s)) of 10. GI untreated animals served as negative controls. The right pouches of GII-GIV animals were painted with 7,12-dimethylbenz (a) anthracene (DMBA) 3 times a week for 14 weeks (ws). While GII received no extra treatment, GIII and GIV received cetuximab intraperitoneally (IP) 3 times a week for 3 ws, and for 6 ws respectively. Gross observations and tumor volume (TV) calculation were obtained after the end experiment. Fresh HBP specimens were surgically bisected into two sections, one for hematoxylin and eosin (H&E) to investigate the depth of invasion (DOI) and the other for immunohistochemistry (IHC) employing monoclonal antibody against epidermal growth factor receptor (EGFR). The other piece was employed for flow cytometry (FCM). Then statistical analysis was imploded.
Results: TV, DOI, EGFR, and SPF aneuploid were highly significantly different between GII and GIV (p = 0.001), while SPF diploid recorded non-significant difference (p>0.608).
Conclusion: The results of the present study indicated that the tumor volume and SPF is a powerful key indicator for tumor activity after treatment of HBP carcinoma by cetuximab.

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Al-Azhar Journal of Dental Science
Volume 27, Issue 3 - Serial Number 3
July - Oral Medicine & Surgical Sciences Issue (Oral Medicine, Oral & Maxillofacial Surgery, Oral Pathology, Oral Biology)
July 2024
Pages 367-377

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