Histological and immunohistochemical evaluation for the role of bone marrow-derived mesenchymal stem cells to counteract the streptozotocin-induced diabetic changes in the rat parotid gland.

Document Type : Review Article

Authors

1 oral biology department - Al-Azhar university

2 Oral and Dental Biology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt

3 Professor, Department of Zoology, Dean of Faculty of Science, Al-Azhar University.

Abstract

Objective: the current study was designed to assess the therapeutic effect of bone marrow-derived mesenchymal stem cells (BMSCs) on parotid glands after streptozotocin induced diabetes in rats. Materials and Methods: 36 Sprague-Dawley rats were divided into 3 groups, 12 rats each. Group I: the animals received 0.1 M sodium citrate buffer intraperitoneally, the vehicle of streptozotocin. GroupII: diabetes mellitus was induced by single intraperitoneal injection of 60 mg/kg of streptozotocin (STZ) dissolved in 0.1 M sodium citrate buffer. Group III: one day after diabetes induction, simgle dose of 2×106 BMSCs suspended in 0.5 ml of phosphate buffer solution (PBS) was injected via intraglandular route. Animals were sacrificed at 7 or 14 days after BMSCs injection, then histological and immunohistochemical studies were done. Result: the experimental stem cell treated group showed better histological features and increased PCNA proliferation more than the untreated group. Conclusion: bone marrow derived stem cell is considered as relatively successful method for the treatment of diabetic induced salivary gland dysfunction.

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Al-Azhar Journal of Dental Science
Volume 27, Issue 3 - Serial Number 3
July - Oral Medicine & Surgical Sciences Issue (Oral Medicine, Oral & Maxillofacial Surgery, Oral Pathology, Oral Biology)
July 2024
Pages 379-388

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