Document Type : Original Article
Authors
1
Assistant lecturer, Oral and Dental Pathology department, Faculty of Dental Medicine, Assiut, Al-Azhar University, Egypt.
2
Assistant Professor, Department of Oral and Dental Pathology Faculty of Dental Medicine, Assiut, Al-Azhar University, Egypt.
3
Professor and head of the Oral and Dental Pathology Department, Faculty of Dental Medicine (Boys Cairo), Al-Azhar University, Egypt.
Abstract
Objective: current research is directed to assess apoptosis induced by capecitabine in combination with graphene oxide (GO) nanoparticles in hamster buccal pouch (HBP) carcinoma. Material & methods: 40 Syrian male hamsters 5 weeks old, weighing 80-120g, had been separated into 4 groups. GI: Animals left untreated. GII: Animals had been painted with 0.5% 7, 12-dimethylbenz (a) anthracene (DMBA) (3 times/week/14weeks). GIII: DMBA was applied followed by administration of capecitabine orally, 600mg/kg/day, daily for a week followed by a week of rest, then a week of treatment. GIV: Animals were painted with DMBA then capecitabine was administered in addition to GO, which was administered by injection directly into the tumor at a dose of 2mg/kg/day, daily for 3 weeks. At end of experiment, animals had been euthanized, then all right pouches were excised, and divided into two parts for further histologic and flowcytometric (FCM) evaluation. Results: Gross observation of GIV confirmed reduction in size of exophytic masses as compared to GIII. Histologic observations of GIV indicated that 5 animals exhibited well-differentiated SCC, 4 animals exhibited severe dysplasia & 1 animal exhibited moderate dysplasia compared to GIII which indicated that 6 animals exhibited well-differentiated SCC & 4 animals exhibited severe dysplasia. FCM detection of apoptosis explained that the percentage of apoptotic cells in GIV had been significantly higher than in GIII. Conclusions: The combination of capecitabine & GO resulted in a synergistic antitumor effect by inhibiting tumor progression and inducing apoptosis more effectively than capecitabine alone in induced HBP carcinoma.
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