Evaluation of pilocarpine potentiality to counteract the rat parotid gland structural alterations induced by gamma irradiation

M. Hani, Said; A.  Elmawla, Abd Elnasser; M. Emran, Ashraf; F. Ahmed, Salwa

doi:10.21608/ajdsm.2018.71527

Evaluation of pilocarpine potentiality to counteract the rat parotid gland structural alterations induced by gamma irradiation

Document Type : Original Article

Authors

¹ Professor of Oral Histology and Pathology, Oral biology department, faculty of dental medicine, Azhar University.

² Assistant Professor of Oral histology, Oral biology department, faculty of dental medicine, Al-Azhar University.

³ Demonstrator of oral histology, Oral Biology Department, faculty of dental medicine, Al-Azhar University

⁴ Lecture in Health Radiation Research Department, National Center for Radiation Research and Technology

10.21608/ajdsm.2018.71527

Abstract

The effect of pilocarpine on irradiation induced rat parotid gland structural alterations was evaluated in 45 adult male Sprague- Dawley rats used in this study and they were divided into three groups,15 rats each. The control group rats did not expose to pilocarpine not radiation. The experimental group II rats were irradiated while those of group III were primarily treated by pilocarpine before irradiation. At 1,4 and 16 weeks after irradiation exposure, 5 rats from each group were euthanasially decapitated. Then the parotid glands were carefully dissected out and prepared for stain by hematoxylin and eosin stain. The histological examination showed that radiation induced harmful effects on the parotid gland were significantly reduced with pilocarpine. Conclusion: Pilocarpine, the parasympathomimetic agonist known to stimulate the various exocrine glands, has considerable improvement for the injurious radiation effects on salivary glands.