Effect of oxygenated water as a new treatment modality on experimentally induced hamster buccal pouch carcinogenesis

Document Type : Original Article

Authors

1 Demonstrator, Oral and Dental Pathology Department, Faculty of Dental Medicine, (Boys- Cairo), Al-Azhar University, Egypt.

2 Assistant Professor, Pharmacology and Toxicology Department, Faculty of Pharmacy, (Boys-Cairo), Al-Azhar University, Egypt.

3 Professor, Oral and Dental Pathology Department, Faculty of Dental Medicine, (Boys-Cairo), Al-Azhar University, Egypt.

Abstract

The aim of the present study was directed to investigate the effect of oxygenated water as a new treatment modality on experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: Eighty-five Syrian male hamsters, five weeks old, weighing 80-120g. The experimental animals were divided into three groups, GI (negative control): 5 animals left untreated. GII {Dimethylbenz[a]anthracene (DMBA) treated group}: 30 animals were painted with 0.5% DMBA in paraffin oil using 3 times a week for 4 weeks (GIIA), 8 weeks (GIIB) and12 weeks (GIIC). GIII (oxygenated water treated group): 45 animals, following each period of DMBA-treatment, were treated in the normal drinking (free access) in addition to once daily obligatory by oral tube for 5 weeks which divided into 3 subgroups GIIIA, GIIIB and GIIIC. Results: Gross observation and histopathological findings revealed a-GI: normal stratified squamous epithelium b- GIIA, GIIIA and GIIIB: mild epithelial dysplasia c: GIIB: moderate and severe epithelial dysplasia d: GIIC and GIIIC: well and moderately differentiated SCC. Immunohistochemical results revealed variable observations among the treated groups (GII and GIII) compared to that observed in group GI. Conclusion: Oxygenated water is considered as a promising treatment agent in preventing of induced HBP carcinogenesis (epithelial dysplasia & invasive carcinoma).

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