Therapeutic potential of carvacrol on experimentally induced hamster buccal pouch epithelial dysplasia

Document Type : Original Article

Authors

1 Demonstrator, Oral and Dental Pathology Department, Faculty of Dental Medicine, Assiut,, Al-Azhar University.

2 Professor, Oral and Dental Pathology Department, Faculty of Dental Medicine, (Boys-Cairo), Al-Azhar University.

3 Professor and Head of Oral and Dental Pathology Department, Faculty of Dental Medicine, Assiut, Al-Azhar University.

Abstract

The aim of the present study was directed to investigate the therapeutic potential of carvacrol on experimentally induced hamster buccal pouch epithelial dysplasia. Material and methods: Seventy golden Syrian male hamsters, five weeks old, weighting 80-120g were divided into three groups(G(s)) GI, GII and GIII. GI (negative control): 10 animals were left untreated. GII: dimethylbenz(a)anthracene (DMBA) induced group): 30 animals were divided into 2 subgroups; GIIA and GIIB. GIIA was painted with 0.5% DMBA in paraffin oil 3 times a week for 8 weeks.GII B was painted with 0.5% DMBA in paraffin oil 3 times a week for 14 weeks. GIII: (carvacrol treated group): 30 animals were divided into 2 subgroups; GIIIA and GIIIB. GIIIA was painted with 0.5% DMBA in paraffin oil 3 times a week for 8 weeks followed by oral administration of carvacrol (15 mg/kg) 3 times a weeks for 6 weeks. GIII B was painted with 0.5% DMBA in paraffin oil 3 times a week for 14 weeks followed by oral administration of carvacrol (15 mg/kg) 3 times a weeks for 6 weeks. Results: Gross observation revealed variation in reduction of the tumor size in the GIII compared to that observed in GII. Histopathological findings in GII revealed variations ranged from moderate dysplasia to well differentiated squamous cell carcinoma while in GIII ranged from mild dysplasia to well differentiated squamous cell carcinoma. Immunohistochemical results revealed that there was highly significant difference between GIIA and GIIIA regarding to Bcl-2, the p value recorded 0.001 (< 0.01) and Bax also showed highly significant difference between the same groups the p value recorded 0.001 (< 0.01). There was highly significant difference between GIIB and GIIIB regarding to Bcl-2, the p value recorded 0.001 (< 0.01) and Bax also showed highly significant difference between the same groups the p value recorded 0.001 (< 0.01). Conclusion: Carvacrol is a novel approach for the treatment of DMBA induced hamster buccal pouch epithelial dysplasia.

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