Document Type : Original Article
Authors
1
Demonstrator, Oral and Dental Pathology Department, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University, Egypt.
2
Lecturer; Department of Oral and Dental pathology, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University, Egypt.
3
Professor and head of Oral and Dental Pathology Department, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University, Egypt.
Abstract
The aim of the present study was directed to investigate the chemopreventive efficacy of carvacrol on experimentally induced hamster buccal pouch(s) (HBP(s) carcinogenesis utilizing Bcl-2 and Bax as apoptotic markers. Material and methods: Fifty five Syrian male hamsters, five weeks old, weighing 80-120 gm. The animals were divided into three groups (G(s)) (GI, GII and GIII): GI (negative control): 5 animals were left untreated. GII: (DMBA painted group): the right BPs of 20 animals were painted with 0.5% DMBA in paraffin oil 3 times a week on alternative days; they were subclassified into two subgroups (GIIA and GIIB). GIIA: the right BPs of 10 hamsters were painted with DMBA for 6 weeks. GIIB: the right buccal pouches of 10 hamsters were painted with DMBA for 14 weeks. GIII (carvacrol chemoprevention group): 30 hamsters were received carvacrol by the oral route using a specific vehicle in a dose of 15mg/kg; they were subclassified into two subgroups (GIIIA and GIIIB). GIIIA: 15 hamsters were given carvacrol one week before, as well as, during DMBA application for 6 weeks. GIIIB: 15 hamsters were given carvacrol one week before, as well as, during DMBA application for 14 weeks. Results: Gross observation revealed that, there is reduction in the size and distribution of tumors in the GIII compared to that observed in GII. Histopathological findings using H&E stain demonstrated variable changes in the GIII ranging from decrease severity to complete absence of dysplastic features compared to those observed in GII. Immunohistochemical (IHC) results of GIII revealed an increased Bax expression and decreased Bcl-2 expression compared to that observed in GII. Conclusion: The incidence of HBP carcinogenesis was prevented and decreased by oral administration of carvacrol with variable degrees, so it might be considered as a promising chemoprevention agent .The positive effect of carvacrol on apoptosis has been realized, as shown by, increased levels of Bax and decreased levels of Bcl-2 expression during carvacrol chemoprevention, in addition to, highly significantly difference of apoptosis between carvacrol chemoprevention group and DMBA painted group. Keywords: HBP carcinogenesis, carvacrol, apoptosis, DMBA.
Keywords
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